• Fundamentals of Instrumental Analysis Methods
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Fundamentals of Instrumental Analysis Methods

Fundamentals of Instrumental Analysis Methods

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The course Fundamentals of Instrumental Analysis Methods covers the evolution of classical methods (gravimetry, volumetric analysis) into fast, sensitive, and automated instrumental techniques. The focus is on optical methods, based on the interaction of electromagnetic radiation with matter, considering the dual nature of light (wave and particle) and its spectrum (UV, visible, infrared) to induce electronic, vibrational, and rotational transitions.

Analytical quantification focuses on radiation absorption, relating transmittance and absorbance, with emphasis on the latter due to its linear scaling with concentration. The course is grounded in the Beer-Lambert Law, which states that absorbance is proportional to concentration, optical path length, and molar absorptivity. Limitations and deviations from the law are also addressed, such as high concentrations, electrostatic interactions, and suspended particles, ensuring the reliability of the calibration curve and the precision of laboratory results.

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